Integrating Proteomics and Metabolomics Approaches to Elucidate the Mechanism of Responses to Combined Stress in the Bell Pepper ( Capsicum annuum )

• Published in: Plants (Basel) Vol. 13; no. 13; p. 1861
• Main Authors: Morales-Merida, Brandon Estefano, Grimaldi-Olivas, Jesús Christian, Cruz-Mendívil, Abraham, Villicaña, Claudia, Valdez-Torres, José Benigno, Heredia, J Basilio, León-Chan, Rubén Gerardo, Lightbourn-Rojas, Luis Alberto, Monribot-Villanueva, Juan L, Guerrero-Analco, José A, Ruiz-May, Eliel, León-Félix, Josefina
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 05.07.2024
• Subjects: Abiotic factors; Abiotic stress; Biosynthesis; Capsicum annuum; Chlorophyll; Climate change; cold; Combined stress; Data integration; Dehydrogenases; DNA methylation; Electron transport; Environmental changes; Enzymes; Flavonoids; Genes; Genomics; Glycosides; integrating omics; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Metabolic pathways; Metabolism; Metabolites; metabolome; Metabolomics; Peppers; Photosynthesis; Physiology; Protein transport; Proteins; proteome; Proteomes; Proteomics; Radiation; Salinity; Scopolamine; Signal transduction; Stems; Ultraviolet radiation; UV-B radiation; Vegetables; Capsicum annuum; cold; UV-B radiation; integrating omics; metabolome; proteome
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants13131861

Bell pepper plants are sensitive to environmental changes and are significantly affected by abiotic factors such as UV-B radiation and cold, which reduce their yield and production. Various approaches, including omics data integration, have been employed to understand the mechanisms by which this crop copes with abiotic stress. This study aimed to find metabolic changes in bell pepper stems caused by UV-B radiation and cold by integrating omic data. Proteome and metabolome profiles were generated using liquid chromatography coupled with mass spectrometry, and data integration was performed in the plant metabolic pathway database. The combined stress of UV-B and cold induced the accumulation of proteins related to photosynthesis, mitochondrial electron transport, and a response to a stimulus. Further, the production of flavonoids and their glycosides, as well as affecting carbon metabolism, tetrapyrrole, and scopolamine pathways, were identified. We have made the first metabolic regulatory network map showing how bell pepper stems respond to cold and UV-B stress. We did this by looking at changes in proteins and metabolites that help with respiration, photosynthesis, and the buildup of photoprotective and antioxidant compounds.