Biological activity of recombinant interleukin-2 in intravenous admixtures containing antibiotic, morphine sulfate, or total parenteral nutrient solution

• Published in: American journal of hospital pharmacy Vol. 49; no. 3; p. 608
• Main Authors: Anderson, P M, Rogosheske, J R, Ramsay, N K, Weisdorf, D J
• Format: Journal Article
• Language: English
• Published: United States 01.03.1992
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - metabolism; Cell Division; Cell Line; Drug Stability; Drug Therapy, Combination; Fat Emulsions, Intravenous - chemistry; Humans; Interleukin-2 - chemistry; Interleukin-2 - metabolism; Morphine - chemistry; Morphine - metabolism; Parenteral Nutrition, Total; Recombinant Proteins - chemistry; Recombinant Proteins - metabolism
• ISSN: 0002-9289
• DOI: 10.1093/ajhp/49.3.608

The biological activity of recombinant interleukin-2 in intravenous admixtures containing gentamicin sulfate, tobramycin sulfate, amikacin sulfate, ticarcillin disodium, piperacillin sodium, morphine sulfate, or total parenteral nutrient (TPN) solution was investigated. Recombinant interleukin-2 in a formulation containing human serum albumin was diluted to a therapeutic dose and mixed with the other drugs in test tubes to simulate mixing distal to the Y-site of an i.v. administration set. All test tubes were visually observed for precipitates. Triplicate test samples of recombinant interleukin-2 and interleukin-2 with drug were added to microplate wells containing lymphocyte tissue culture media (an interleukin-2-dependent murine cytotoxic T cell line, CTLL-2) using serial dilutions. The microplates were incubated then pulsed with mouse lymphocyte media containing tritiated thymidine (3H-thymidine). The amount of 3H-thymidine incorporated into lymphocyte DNA was measured using a beta counter. The average counts per minute (cpm) of a sample was analyzed as a function of the logarithm of sample dilution; one interleukin-2 unit was defined as 50% of the maximum cpm measured in the samples. At minimum dilutions, ticarcillin disodium reduced the effect of interleukin-2 on CTLL-2 growth; the other antibiotics and morphine sulfate had no effect. Interleukin-2 mixed with TPN solutions showed slightly enhanced growth of the CTLL-2 line compared with the interleukin-2 standard. Mixtures of multiple antibiotics, morphine, and TPN solutions with and without 20% fat emulsion had no effect on interleukin-2 activity.