The determination of basal glycemia and glucose tolerance curve: the manners of blood sampling

• Published in: Acta medica Lékarskí fakulty Univerzity Karlovy v Hradci Králove Vol. 41; no. 3; pp. 121 - 123
• Main Author: Golda, V
• Format: Journal Article
• Language: English
• Published: Czech Republic Karolinum Press 1998
• Subjects: Anesthesia; Animals; Basal glycemia; Blood Glucose - analysis; Blood sampling; Blood Specimen Collection - methods; Ether; Ether anaesthesia; Glucose Tolerance Test; GTT; Nembutal anaesthesia; Pentobarbital; Rats; Rats, Inbred SHR; Rats, Wistar; Retrobulbar plexus
• ISSN: 1211-4286; 1805-9694
• DOI: 10.14712/18059694.2019.175

There were analyzed the conditions under which the basal glycemia and/or glucose tolerance curve is not contaminated by stress induced changes in glycide metabolism. When the basal glycemia was monitored, blood was sampled to heparinized capillaries from retrobulbar plexus under the light ether anaesthesia or by decapitation without narcosis. The animal represented the control for itself. No differences was found in basal glycemia under the two mentioned blood sampling. In the second series of experiments glycemia was monitored in the time schedule which is used in glucose tolerance test, i.e., blood sampling was performed 30, 60, 120 and 180 min after the first sampling from retrobulbar plexus either under light ether anaesthesia (in 14 h starvated rats or in rats with free access to diet) or under Nembutal anaesthesia (in 14 h starvated rats). No differences were found in glycemia when two types of narcosis is compared. No signs of augmentation were detected. In the last series when blood sampling was taken in two sec intervals, time dependent augmentation of stress glucose elevation was found. The augmentation was more expressed in the rats with free access to diet than in starvated animals.