An Update of Oocyte Vitrification: A Modification of Sucrose and Trehalose as Extracellular Cryoprotectant

As well as the development of assisted reproductive technology (ART), as the current treatment of woman who failed in achieving pregnancy, the development of an advance vitrification method also grows rapidly. The successful of oocyte vitrification depends on the type and the concentration of cryopr...

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Bibliographic Details
Published inBiomedical & pharmacology journal Vol. 11; no. 1; pp. 209 - 214
Main Authors Lestari, Silvia W, Fitriyah, Nurin N, Margiana, Ria
Format Journal Article
LanguageEnglish
Published Bhopal Oriental Scientific Publishing Company 01.03.2018
Biomedical and Pharmacology Journal
Subjects
Cooling
Cryopreservation
Cryoprotectors
Embryos
Intracellular
Methods
Microinjection
Pregnancy
Reproductive technology
Sheep
Studies
Success
Sucrose
Sugar
Survival
Systematic review
Technology
Trehalose
Vitrification
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Summary:As well as the development of assisted reproductive technology (ART), as the current treatment of woman who failed in achieving pregnancy, the development of an advance vitrification method also grows rapidly. The successful of oocyte vitrification depends on the type and the concentration of cryoprotectant. This study was addressed to elaborate empirical evidence and recent studies of sucrose and trehalose as an extracellular CPA with the aim of achieving the success of oocyte vitrification. Several researchers in agreement that trehalose, as extracellular cryoprotectant, also has a role as intracellular cryoprotectant by microinjection with high survival rates as the outcome. Moreover, the combination of sucrose or trehalose as an extracellular cryoprotectant and others intracellular cryoprotectant have different survival rates which might occur because of the differences between the composition and concentration of sucrose or trehalose. The appropriate type and concentration of sugar as an extracellular cryoprotectant for oocyte cryopreservation are sucrose or trehalose in 0.5M concentration. Nevertheless, it requires further study to optimize oocyte vitrification process.
ISSN:0974-6242
2456-2610
DOI:10.13005/bpj/1365