Identification and antioxidant activity of phenolic compound from leaves of Scurrula parasitica L

• Published in: Journal of physics. Conference series Vol. 1397; no. 1; pp. 12024 - 12030
• Main Authors: Atun, Sri, Nur Innayati, Asri, Herlambang, HW, Handayani, Sri
• Format: Journal Article
• Language: English
• Published: Bristol IOP Publishing 01.12.2019
• Subjects: Antioxidants; Chloroform; Ethanol; Ethyl acetate; Hexanes; NMR; Nuclear magnetic resonance; Phenols; Physics; Recrystallization; Solvents; Spectrophotometers; Thin layer chromatography
• ISSN: 1742-6588; 1742-6596
• DOI: 10.1088/1742-6596/1397/1/012024

Scurrula parasitica L is one of the Loranthus plant species which is an herbal plant recorded in ancient documents and widely used as traditional medicine. The purpose of this study was to identify phenolic compounds and to test the antioxidant activity of compounds from Scurrula parasitica L. leaf extract. The research method was carried out by extracting dried powder of S. parasitica leaves with ethanol. Ethanol extract was partitioned using n-hexane, chloroform, and ethyl acetate solvents respectively. The ethyl acetate fraction obtained is then concentrated to form deposits. The precipitate obtained was separated and washed using n-hexane and a mixture of ethyl acetate: acetone (1: 1) in sequence. The precipitate obtained is then purified by recrystallization with ethanol as solvent. The crystals obtained were tested for their purity by thin layer chromatography (TLC) and identified using UV-Vis, IR and NMR spectrophotometers. The method for testing antioxidant activity using 2, 2- diphenyl-1-picrylhydrazyl (DPPH). The results of spectroscopic identification showed that the isolation compound obtained was quercitrin (quercetin-3-O-rhamnoside). Quercitrin shows high antioxidant activity with IC50 5.00 μg / mL. Thus, S. parasitica plant is very potential as a source of natural antioxidants