Assessment of the antibacterial effect of Khaya senegalensis on some Gram-negative bacteria

• Published in: Bulletin of the National Research Centre Vol. 45; no. 1; pp. 1 - 8
• Main Authors: Dougnon, Victorien, Hounsa, Edna, Koudokpon, Hornel, Agbodjento, Eric, Afaton, Anny, Sintondji, Kevin, Klotoe, Jean Robert, Segbo, Julien, Baba-Moussa, Lamine
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 08.06.2021; Springer Nature B.V; SpringerOpen
• Subjects: Antibacterial activity; Antibiotics; Antimicrobial resistance; Bacteria; Cefixime; Destabilization; Diarrhea; E coli; Ethanol; Gram-negative Bacteria; Humanities and Social Sciences; Khaya senegalensis; Membrane permeability; Membranes; Microorganisms; Minimum inhibitory concentration; Mode of action; Molecular Biology and Biotechnology; multidisciplinary; Salmonella; Science; Science (multidisciplinary); Stability; Strains (organisms); Gram-negative Bacteria; Antibacterial activity; Mode of action
• ISSN: 2522-8307; 2522-8307
• DOI: 10.1186/s42269-021-00568-0

Background The matter of antimicrobial resistance evokes the urgency to explore alternatives to the antibiotics traditionally used for microbial infections. This study aimed to elucidate the mechanism of action of the antibacterial effect of Khaya senegalensis liable for bacterial strains responsible for diarrheal infections. Results The data collected indicate that the bacterial strains tested ( Salmonella Typhimurium ATCC 14028, Escherichia coli ATCC 25922, Shigella spp. and Salmonella spp.) were sensitive to the extracts of Khaya senegalensis (Desr.) A.Juss. (aqueous and hydro-ethanol) to varying degrees. The hydro-ethanolic extract was active on all strains with a MIC of 25 mg/mL coupled with a bactericidal effect. The aqueous extract was only active on the Salmonella spp. strain. Membrane permeability test data show that Khaya senegalensis extracts affect the bacterial strains tested by attacking the stability of their outer membrane. This potential indicated by the high percentage of membrane destabilization of the bacteria is significantly ( p  < 0.05) better than that of cefixime used as a reference. Conclusion This study revealed that Khaya senegalensis destroys Gram-bacteria by attacking the stability of their cytoplasmic membrane. These data provide for the first time the mode of action of Khaya senegalensis extracts concerning their antibacterial activity.