Rapid detection for sporeless trait from Pleurotus pulmonarius culture extracts by using real-time PCR

The release and spread of a large number of basidiospores from the developing fruiting body cause serious problems in the cultivation of edible mushrooms, including Pleurotus pulmonarius (Fr.) Quel. The P. pulmonarius sporeless mutant, TMIC-30058, has a high potential for breeding sporeless cultivar...

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Bibliographic Details
Published inMycoscience Vol. 52; no. 2; pp. 143 - 146
Main Authors Okuda, Yasuhito, Matsumoto, Teruyuki, Ninomiya, Kenji
Format Journal Article
LanguageEnglish
Published The Mycological Society of Japan 2011
Subjects
Marker-assisted selection
Mushroom breeding
Oyster mushroom
Sporulation-deficient mutation
STS marker
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Summary:The release and spread of a large number of basidiospores from the developing fruiting body cause serious problems in the cultivation of edible mushrooms, including Pleurotus pulmonarius (Fr.) Quel. The P. pulmonarius sporeless mutant, TMIC-30058, has a high potential for breeding sporeless cultivars to reduce these adverse effects. Our previous study (Okuda et al. in Breeding Science 59:315–319, 2009) showed two sequence-tagged site (STS) markers for the sporeless trait from this mutant. Here, using these STS markers we present rapid detection of sporeless trait from P. pulmonarius culture extracts by real-time PCR. This method enables us to cut down time and labor for screening of the sporeless trait, suggesting its availability for efficient breeding of sporeless cultivars.
ISSN:1340-3540
1618-2545
DOI:10.47371/S10267-010-0078-Z