Portable, High-Sensitive, and Metglas Alloy 2826 MB-Based Magnetoelastic Biosensor to Detect the Osteoarthritis Marker MMP-3

• Published in: IEEE sensors journal Vol. 22; no. 24; pp. 23780 - 23785
• Main Authors: Guo, Xing, Li, Hongmei, Hou, Jianru, Li, Pengcui, Li, Haoqian, Guo, Li, Che, Xianda, Yuan, Zhongyun, Sang, Shengbo
• Format: Journal Article
• Language: English
• Published: New York The Institute of Electrical and Electronics Engineers, Inc. (IEEE) 15.12.2022
• Subjects: Antibodies; Antigens; Arthritis; Biomarkers; Biosensors; Diagnosis; Ferrous alloys; Frequency shift; Magnetostriction; Matrix metalloproteinases; Osteoarthritis; Resonant frequencies
• ISSN: 1530-437X; 1558-1748
• DOI: 10.1109/JSEN.2022.3220323

Osteoarthritis (OA) is one of the most common joint disorders affecting human health. Early detection of OA is essential for effective treatment of the disease. There is a growing demand of techniques for highly sensitive detection of trace OA markers. Here, the magnetoelastic (ME) biosensor based on Metglas alloy 2826 MB is presented to detect matrix metalloproteinase-3 (MMP-3) of the OA biomarker, through the measurement of resonant frequency changes with excellent magnetostrictive effects. The detection system consists of an ME chip immobilized with MMP-3 antibody and an electromagnetic coil. Since the ME biosensor is ultrasensitive to mass changes, the frequency shift caused by mass changes induced by antibody–antigen-specific binding can be used to reflect the amount of MMP-3. The ME biosensor has been applied to detect the actual joint fluid sample of OA patients, demonstrating its feasibility. The results show that this assay has a linear response to the MMP-3 concentrations ranging from 30.7 to [Formula Omitted] with a detection limit of [Formula Omitted], covering the range of clinical tests required for the diagnosis of OA. The ME biosensor provides a novel and viable method for the diagnosis of early OA.