The fertility tracks of pollen tube in the ovary of Solanum nigrum by three-dimensional reconstruction

• Published in: Journal of microscopy (Oxford) Vol. 293; no. 2; pp. 86 - 97
• Main Authors: Ming, Xing, Chen, Xia, Ma, Hongyu, Li, Chuang, Zhao, Zijian, Li, Jinying, Du, Yingda
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.02.2024
• Subjects: Fertility; Fertilization; Flowers - anatomy & histology; Germination; Imaging, Three-Dimensional; Ovaries; Ovary; Ovules; Pollen; Pollen Tube; Pollen tubes; Pollination; Reconstruction; Seeds; Solanum nigrum; Solanum nigrum; 3D reconstruction; pollen tube; fertility
• ISSN: 0022-2720; 1365-2818
• DOI: 10.1111/jmi.13257

In this paper, we present an enhanced method for automatically capturing a large number of consecutive paraffin sections using a microscope. Leveraging these microstructural images, we employed three-dimensional visualisation and reconstruction techniques to investigate the dispersal growth process of pollen tube bundles upon entering the ovary of Solanum nigrum. Additionally, we explored their behaviour within different ovules and examined the relationship between the germination rate of seeds and the fertilisation process. Our findings reveal that despite the abundance of Solanum nigrum seeds, only a fraction of them is capable of successful germination. The germination rate of seeds is closely related to whether fertilisation of the ovules and pollen tubes is completed. Due to the limited number of pollen tubes entering the ovary, only a portion of the ovules can be fertilised. The proportion of fertilised ovules positively correlates with the germination rate of the seeds. Through three-dimensional reconstruction, we observed a phenomenon of proximity during the pollination process, wherein ovules closer to the pollen tube bundles are more likely to be fertilised. Furthermore, fertilised ovules exhibited significant changes in morphology and embryo sac structure. The number of fertilised ovules directly impacts the germination rate of wild Solanum nigrum seeds. Although all Solanum nigrum ovules have the potential to develop into seeds, most seeds originating from unfertilised ovules are unable to germinate normally, resulting in an incomplete germination rate of seeds and preventing it from reaching 100%.