Stimulation of human immunodeficiency virus expression in permanent monocytic cells by Sarcocystis gigantea extract

As recently reported, a strong stimulation of noninfected CD4+ H9- cells by Sarcocystis gigantea (syn. S. ovifelis) extract (SGE) was observed using the lymphocyte proliferation assay. After SGE prestimulation, human immunodeficiency virus (HIV)-infected H9+ cells showed an exacerbation of viral rep...

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Published inParasitology research (1987) Vol. 84; no. 6; pp. 455 - 458
Main Authors DROSSIGK, U, HIEPE, T, POTZSCH, F, SCHOLZ, D, TIETZ, H.-J
Format Journal Article
LanguageEnglish
Published Berlin Springer 01.06.1998
Subjects
AIDS/HIV
Animals
Biological and medical sciences
Cell Extracts - pharmacology
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
General aspects and techniques
HIV Core Protein p24 - analysis
HIV-1 - physiology
Humans
Microbiology
Monocytes - virology
Protozoa
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Sarcocystis
Tumor Cells, Cultured
Virology
Virus Replication
Human
Protozoa
Apicomplexa
Microorganism interrelationships
Virus multiplication
Monocyte
Retroviridae
Cell extract
Activation
Lentivirus
In vitro
Virus
Cell line
Microbial origin
Infected cell
Sarcocystis
Human immunodeficiency virus
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Summary:As recently reported, a strong stimulation of noninfected CD4+ H9- cells by Sarcocystis gigantea (syn. S. ovifelis) extract (SGE) was observed using the lymphocyte proliferation assay. After SGE prestimulation, human immunodeficiency virus (HIV)-infected H9+ cells showed an exacerbation of viral replication. In the present study we investigated the reactivity of HIV-infected human monocytes using SGE. The highly sensitive p24 core-profile enzyme-linked immunosorbent assay (ELISA) was used to examine directly the amount of HIV produced. Experiments were performed using U937 permanent monocytic cells. Permanent incubation as well as preincubation with SGE before virus infection stimulated HIV expression in all the cells. In U937 cells the viral release per cell was 64 times higher on permanent stimulation with 320 microg SGE relative to controls and 9 times higher following prestimulation.
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ISSN:0932-0113
1432-1955
DOI:10.1007/s004360050429