Reaction sequences from light absorption to the cleavage of water in photosynthesis : Routes, rates and intermediates

• Published in: Photosynthesis research Vol. 10; no. 3; p. 453
• Main Authors: Witt, H T, Schlodder, E, Brettel, K, Saygin, O
• Format: Journal Article
• Language: English
• Published: Netherlands 01.01.1986
• ISSN: 0166-8595; 1573-5079
• DOI: 10.1007/BF00118312

The reaction sequence between the primary electron acceptor, the oxidized Chlorophyll-aII, and the terminal electron donor, the water splitting enzyme system S, is being described in the range from nanoseconds to milliseconds. For the cleavage of water Chlorophyll-aII (+) extracts four electrons in four turnovers from the enzyme system S responsible for the water oxidation. For each extraction the electron is moved step by step along the chain that connects the Chlorophyll-aII center with that of S. Beginning with the transfer from the immediate donor, D1, to Chl-aII (+), the subsequent transfer from D2 to D1 (+) ends in the electron transfer from S to D2 (+). This final act establishes in S the oxidizing equivalent, probably in the form of oxidized manganese. Coupled with these acts is an intrinsic proton release and a surplus charge formation. After the generation of the 4th oxidizing equivalent in a concerted final action the evolution of O2 from water takes place. Correlations between the events are described quantitatively.