On-Channel Micro-Solid Phase Extraction Bed Based on 1-Dodecanethiol Self-Assembly on Gold-Deposited Colloidal Silica Packing on a Capillary Electrochromatographic Microchip

A fully packed capillary electrochromatographic (CEC) microchip with an on-column micro-solid phase extraction (SPE) bed for the preconcentration and separation of organic analytes was prepared. A linear microchannel with monodisperse colloidal silica packing was formed on a cyclic olefinic copolyme...

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Published inBulletin of the Korean Chemical Society Vol. 35; no. 1; pp. 45 - 50
Main Authors Park, Jongman, Kim, Shinseon
Format Journal Article
LanguageEnglish
Published 대한화학회 20.01.2014
Subjects
화학
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Summary:A fully packed capillary electrochromatographic (CEC) microchip with an on-column micro-solid phase extraction (SPE) bed for the preconcentration and separation of organic analytes was prepared. A linear microchannel with monodisperse colloidal silica packing was formed on a cyclic olefinic copolymer microchip with two reservoirs on both ends. Silver-cemented silica packing frit structure was formed at the entrance of the microchannel by electroless plating treatment as a base layer. A gold coating was formed on it by reducing Au 3+ to gold with hydroxylamine. Finally micro-SPE bed was formed by self-assembly adsorption of 1- dodecanethiol on it. Micro-SPE beds were about 100-150 m long. Approximately 10 3 fold sensitivity enhancements for Sulforhodamine B, and Fluorescein in nM concentration levels were possible with 80 s preconcentration. Basic extraction characteristics were studied. KCI Citation Count: 0
Bibliography:http://journal.kcsnet.or.kr/main/j_search/j_abstract_view.htm?code=B140107&qpage=j_search&spage=b_bkcs&dpage=ar
G704-000067.2014.35.1.009
ISSN:0253-2964
1229-5949
DOI:10.5012/bkcs.2014.35.1.45