Morphological and molecular characterisation of Hemicriconemoides paracamelliae sp. n. (Nematoda: Criconematidae) and two known species of Hemicriconemoides from China

• Published in: Nematology : international journal of fundamental and applied nematological research Vol. 20; no. 5; pp. 403 - 422
• Main Authors: Maria, Munawar, Cai, Ruihang, Castillo, Pablo, Zheng, Jingwu
• Format: Journal Article
• Language: English
• Published: Leiden Brill Academic Publishers, Inc 2018
• Subjects: Annuli; Anus; Ectoparasites; Elongation; Flaps; Hemicriconemoides; Molecular chains; Morphology; Nematodes; New species; Pharynx; Physical characteristics; Rhizosphere; Spermatheca; Vulva; China
• ISSN: 1388-5545; 1388-5545; 1568-5411
• DOI: 10.1163/15685411-00003147

Summary Sheathoid nematodes of the genus Hemicriconemoides are migratory root-ectoparasites of many crops but damage is documented for only a few species. Hemicriconemoides paracamelliae sp. n., isolated in Hangzhou, Zhejiang, China, from the rhizosphere of Cinninghamia lanceolata , is described together with H. kanayaensis and H. parataiwanensis . These three species are characterised morphologically and molecularly with important morphological details elucidated by SEM photographs. The new species can be characterised by the en face view having a dorsoventrally orientated oral disc with slit-like opening, and the labial plate being composed of two lateral semi-globular shaped projections on the lateral sides of the oral disc. This new species has the first lip annulus expanded and slightly wider than the second, stylet 83 (80-85) μ m long, excretory pore located 5-6 annuli posterior to the base of the pharyngeal bulb, vulva slit-like, vulval flaps absent, spermatheca rounded to oblong-shaped, and anus located 5-8 annuli posterior to vulva. The tail is elongated, conoid, with the terminal annuli curving dorsally or ventrally to form a finely rounded tip. The study provides the first record of H. kanayaensis from mainland China, a new host association of H. parataiwanensis , and molecular sequencing data of the 18S, 28S D2-D3 and ITS sequences.