Development of the innovative biotechnological methods of the biological preparations production for feed purposes

• Published in: IOP conference series. Earth and environmental science Vol. 1052; no. 1; pp. 12016 - 12020
• Main Authors: Volkova, G S, Sokolova, E N, Fursova, N A, Yuraskina, T V, Serba, E M
• Format: Journal Article
• Language: English
• Published: Bristol IOP Publishing 01.07.2022
• Subjects: Amino acids; Biomass; Enzymes; Flour; Growth rate; Peas; Saccharomyces cerevisiae; Titration; Yeast; Yeasts
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/1052/1/012016

Abstract The aim of this study was the production of the biological preparations based on the hydrolyzed pea flour and the yeast biomass. The research objects were Saccharomyces cerevisiae Y-1218 yeast with glucoamylase activity, pea flour, and Celloviridin S20X and Amyloprotoorizin S20X enzyme preparations (EP). The specific growth rate of the yeast was determined by the calculation method, protein content – via Kjeldahl and Lowry methods, reducing substances content – by Somogyi-Nelson assay, the amine nitrogen content – by the formol titration method, the number of living yeast cells – by sowing of dilutions. The amino acid content was measured on the KNAUER (Germany) high-performance liquid chromatograph. As the result of the studies, enzyme preparations for the fermentative processing of pea flour were selected. The cultivation conditions of the Saccharomyces cerevisiae Y-1218 yeast were opted. Under these chosen growing parameters the produced biomass contained 2.4×10 9 CFU/cm 3 and specific growth rate of yeast was 0.264 h -1 . The resulting biomass contained a high amount of essential amino acids. The biopreparations production based on the described ingredient is perspective. These biopreparations with increased biological value could be used for feed purposes.