Finding consensus miRNAs silencing KLF1 expression as a promising therapeutic option of sickle cell anemia

Sickle cell anemia (SCA) is the most severe hemoglobinopathy with no known cure till date. Patients with SCA have a shorter lifespan and suffer from painful crises as well as end-organ damages. The goal of this in silico work is to find the consensus miRNAs targeting Kruppel-like factor (KLF1) gene,...

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Bibliographic Details
Published inInformatics in medicine unlocked Vol. 39; p. 101272
Main Authors Al-Madhagi, Haitham Ahmed, Shahab, Muhammad
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 2023
Elsevier
Subjects
Gene therapy
Hybridization energy
KLF1
miRNA
Sickle cell anemia
Hybridization energy
miRNA
Sickle cell anemia
Gene therapy
KLF1
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Summary:Sickle cell anemia (SCA) is the most severe hemoglobinopathy with no known cure till date. Patients with SCA have a shorter lifespan and suffer from painful crises as well as end-organ damages. The goal of this in silico work is to find the consensus miRNAs targeting Kruppel-like factor (KLF1) gene, responsible for HbF-to-HbA switching. Three publicly available databases were searched, miRDB, miRWalk and TargetScan. Afterwards, the hybridization examination of the predicted miRNAs was evaluated. Docking to human argonaute-1 and the subsequent molecular dynamics simulation were also conducted. In conclusion, hsa-miR-330–5p was the best miRNA targeting KLF1 gene in almost all aspects providing a potential avenue for the development of effective SCA treatments. Further validation in vitro is warranted to fully explore the therapeutic potential of this miRNA candidate.
ISSN:2352-9148
2352-9148
DOI:10.1016/j.imu.2023.101272