Gordonia zhenghanii sp. nov. and Gordonia liuliyuniae sp. nov., isolated from bat faeces

• Published in: International journal of systematic and evolutionary microbiology Vol. 72; no. 10
• Main Authors: Huang, Yuyuan, Gong, Jian, Dong, Lingzhi, Yang, Jing, Lu, Shan, Lai, Xin-He, Pu, Ji, Jin, Dong, Xu, Jianguo
• Format: Journal Article
• Language: English
• Published: 01.01.2022
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.005579

Four mesophilic actinobacteria (HY002 T , HY442, HY366 T and HY285) isolated from the faeces of bats collected in southern China were found to be strictly aerobic, non-motile, rod-shaped, oxidase-negative, Gram-stain-positive and catalase-positive. Strains HY002 T and HY366 T contained meso -diaminopimelic acid as the diagnostic diamino acid and MK-9(H 2 ) the sole respiratory quinone. Arabinose, galactose and ribose were detected in the whole-cell hydrolysates of both type strains. The main cellular fatty acids (> 10.0%) of all strains were C 16 : 0 , C 18 : 1  ω 9 c , 10-methyl-C 18 : 0 and summed feature 3. Strains HY002 T and HY366 T contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidyl inositol mannosides as the major polar lipids. The phylogenetic/phylogenomic analyses based on 16S rRNA gene and genomic sequence comparison revealed that the four strains belong to the genus Gordonia , most closely related to G. neofelifaecis NRRL B-59395 T (98.2–98.3% sequence similarity) on the EzBioCloud database. The G+C contents of strains HY002 T and HY366 T based on genomic DNA were 66.5 and 66.9%, respectively. The DNA–DNA relatedness values between the two types strains and members of the genus Gordonia were far below 70 % (18.6–23.1 %). All genotypic and phenotypic data indicated that the four strains are representatives of two novel separate species, for which the names Gordonia zhenghanii sp. nov. and Gordonia liuliyuniae sp. nov. are proposed, with HY002 T (=CGMCC 4 7757 T =JCM 34 878 T ) and HY366 T (=CGMCC 1 19146 T =JCM 34 879 T ) as the respective type strains.