A simple method for the in vitro determination of phagocytic activity of bovine polymorphonuclear leukocytes

A simple method for the in vitro determination of the phagocytic activity of bovine polymorphonuclear leukocytes (PMNL) is described. An enriched PMNL population was obtained from peripheral blood of healthy cattle and mixed with opsonized Candida utilis or with opsonized bovine red blood cells on B...

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Published inVeterinary immunology and immunopathology Vol. 18; no. 2; pp. 139 - 147
Main Authors Soler-Rodríguez, A M, Armas, J M
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.03.1988
Subjects
Animals
Cattle
Cell Adhesion
Cell Separation
Cell Survival
Erythrocytes - immunology
Immune Sera - pharmacology
Immunologic Tests - methods
Leukocyte Count
Male
Neutrophils - immunology
Neutrophils - physiology
Phagocytosis
Rabbits
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Summary:A simple method for the in vitro determination of the phagocytic activity of bovine polymorphonuclear leukocytes (PMNL) is described. An enriched PMNL population was obtained from peripheral blood of healthy cattle and mixed with opsonized Candida utilis or with opsonized bovine red blood cells on BSA treated coverglass slides. The phagocytosis and killing of C. utilis was determined simultaneously on the coverglass slides stained with acridine orange. Phagocytosis of bovine erythrocytes was determined by microscopic examination of the Wright-Giemsa stained slides. This method is appropriate under limited laboratory conditions because it does not require highly sophisticated equipment and materials, it is easy and rapid to perform, reproducible and inexpensive. Therefore, it could be used as a first evaluation of the phagocytic activity of bovine PMNL.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0165-2427
1873-2534
DOI:10.1016/0165-2427(88)90056-6