Nocardioides malaquae sp. nov., a novel actinobacterium isolated from sewage sludge of a fisheries processing factory

• Published in: International journal of systematic and evolutionary microbiology Vol. 71; no. 7
• Main Authors: Zhang, Xinyin, Wu, Zhicheng, Yan, Cen, Chen, Can, Zheng, Gang, Ye, Yanghui, Sun, Cong, Wu, Min
• Format: Journal Article
• Language: English
• Published: 01.01.2021
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.004852

A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain (Y6 T ) was isolated from a sewage sludge sample collected from a fisheries processing factory in Zhoushan, Zhejiang Province, PR China. The growth range of NaCl concentration was 0–6.0 % (w/v), with an optimum at 3.0 % (w/v). The temperature range for growth was 10–42 °C, with an optimum at 37 °C. The pH range for growth was pH 7.0–10.0, with an optimum at pH 9.0. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Y6 T belonged to the genus Nocardioides and showed the highest sequence similarity of 97.8 % to Nocardioides jishulii dk3136 T . The average nucleotide identity and in silico DNA–DNA hybridization values between strain Y6 T and the reference strains were 76.9–81.2 % and 20.6–23.6 %, respectively. Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-8(H 4 ) and the predominant cellular fatty acids were iso-C 16 : 0 , 10-methyl-C 17 : 0 and C 18 : 1  ω 9 c . The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, four unidentified phospholipids, three unidentified aminolipids and five unidentified lipids. The peptidoglycan was ll -2,6-diaminopimelic acid. On the basis of the phenotypic, genotypic, phylogenetic and chemotaxonomic features, strain Y6 T is considered to represent a novel species, for which the name Nocardioides malaquae sp. nov. is proposed. The type strain is Y6 T (=KCTC 49504 T =MCCC 1K04765 T ).