A study of the pairing interaction between protein subunits in the tobacco mosaic virus family by image reconstruction from electron micrographs

Three-dimensional image reconstruction techniques have been applied to electron micrographs of three related structures, the dahlemense strain of tobacco mosaic virus, the RNA-free protein helix of the same strain and the protein helix of the vulgare strain. Three-dimensional models of the structure...

Full description

Saved in:
Bibliographic Details
Published inJournal of molecular biology Vol. 92; no. 4; pp. 541,IN15,547 - 546,IN20,558
Main Authors Sperling, R., Amos, L.A., Klug, A.
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 15.03.1975
Subjects
Methods
Microscopy, Electron
Models, Structural
Optics and Photonics
Protein Conformation
Tobacco Mosaic Virus - analysis
Tobacco Mosaic Virus - ultrastructure
Viral Proteins
X-Ray Diffraction
Online AccessGet full text

Cover

More Information
Summary:Three-dimensional image reconstruction techniques have been applied to electron micrographs of three related structures, the dahlemense strain of tobacco mosaic virus, the RNA-free protein helix of the same strain and the protein helix of the vulgare strain. Three-dimensional models of the structures are compared. The arrangement of subunits in all three cases is shown to be regularly perturbed in such a way that adjacent turns of the basic helix are paired, but the strength and nature of the pairing interaction varies somewhat from one structure to another. The significance of these differences is discussed in relation to the role of the pairing interaction between subunits.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-2836
1089-8638
DOI:10.1016/0022-2836(75)90308-3