Survival of synchronized Chinese hamster cells following freezing in liquid nitrogen

• Published in: Experimental cell research Vol. 63; no. 2; pp. 476 - 477
• Main Authors: Koch, G.J., Kruuv, J., Bruckschwaiger, C.W.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.1970
• Subjects: Animals; Cell Division; Cricetinae; Culture Techniques; Freezing; Methods; Nitrogen

Synchronized Chinese hamster cells were frozen in tissue culture medium with 15% glycerol added to −196 °C at various points in the cell cycle. The highest survival was obtained with cells frozen in M and late S, while the lowest survival was obtained with cells in G2. These differed by a factor of...