Survival of synchronized Chinese hamster cells following freezing in liquid nitrogen

Synchronized Chinese hamster cells were frozen in tissue culture medium with 15% glycerol added to −196 °C at various points in the cell cycle. The highest survival was obtained with cells frozen in M and late S, while the lowest survival was obtained with cells in G2. These differed by a factor of...

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Bibliographic Details
Published inExperimental cell research Vol. 63; no. 2; pp. 476 - 477
Main Authors Koch, G.J., Kruuv, J., Bruckschwaiger, C.W.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.12.1970
Subjects
Animals
Cell Division
Cricetinae
Culture Techniques
Freezing
Methods
Nitrogen
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Summary:Synchronized Chinese hamster cells were frozen in tissue culture medium with 15% glycerol added to −196 °C at various points in the cell cycle. The highest survival was obtained with cells frozen in M and late S, while the lowest survival was obtained with cells in G2. These differed by a factor of 2.2.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0014-4827
1090-2422
DOI:10.1016/0014-4827(70)90245-4