Testing the mutagenic potency of chemical substances in a linear host-mediated assay I. Experimental microbiological basis
By the use of the mutagenic substance hydrazine sulphate it is shown that the currently used single determination method for determining point mutations in host-mediated assays with calculation of mutation frequencies can lead to erroneous results. The microbiological basis for a linear method is pr...
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| Published in | Mutation Research/Environmental Mutagenesis and Related Subjects Vol. 31; no. 4; pp. 205 - 216 |
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| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
Netherlands
Elsevier B.V
01.08.1975
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0165-1161 0027-5107 |
| DOI | 10.1016/0165-1161(75)90002-3 |
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| Abstract | By the use of the mutagenic substance hydrazine sulphate it is shown that the currently used single determination method for determining point mutations in host-mediated assays with calculation of mutation frequencies can lead to erroneous results. The microbiological basis for a linear method is presented in which the population growth of the auxotrophic and substance-induced mutants used in the test can be described mathematically during their logarithmic growth phase with regression lines. |
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| AbstractList | By the use of the mutagenic substance hydrazine sulphate it is shown that the currently used single determination method for determining point mutations in host-mediated assays with calculation of mutation frequencies can lead to erroneous results. The microbiological basis for a linear method is presented in which the population growth of the auxotrophic and substance-induced mutants used in the test can be described mathematically during their logarithmic growth phase with regression lines. By the use of the mutagenic substance hydrazine sulphate it is shown that the currently used single determination method for determining point mutations in host-mediated assays with calculation of mutation frequencies can lead to erroneous results. The microbiological basis for a linear-method is presented in which the population growth of the auxotrophic and substance-induced mutants used int he test can be described mathematically during their logarithmic growth phase with regression lines. |
| Author | Vollmar, Joachim Grafe, Alfred Lorenz, Rolf |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/1095922$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1007/BF00332388 10.1007/BF00277360 10.1007/BF00271647 10.1016/0027-5107(73)90214-5 |
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| Copyright | 1975 |
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| References | Legator (BIB3) 1970 Ray, Holden, Ellis, Hyneck (BIB6) 1973; 18 Garbridge, Legator (BIB1) 1969; 130 Kaplan, Brendel (BIB2) 1969; 104 World (BIB7) 1971 Propping, Buselmaier (BIB4) 1971; 28 Propping, Röhrborn, Buselmaier (BIB5) 1972; 117 Propping (10.1016/0165-1161(75)90002-3_BIB4) 1971; 28 Ray (10.1016/0165-1161(75)90002-3_BIB6) 1973; 18 Garbridge (10.1016/0165-1161(75)90002-3_BIB1) 1969; 130 Propping (10.1016/0165-1161(75)90002-3_BIB5) 1972; 117 Legator (10.1016/0165-1161(75)90002-3_BIB3) 1970 Kaplan (10.1016/0165-1161(75)90002-3_BIB2) 1969; 104 World (10.1016/0165-1161(75)90002-3_BIB7) 1971 |
| References_xml | – volume: 28 start-page: 129 year: 1971 end-page: 134 ident: BIB4 article-title: The influence of metabolism on mutagenic activity in the host-mediated assay publication-title: Arch. Toxikol. – volume: 117 start-page: 197 year: 1972 end-page: 209 ident: BIB5 article-title: Comparative investigations on the chemical induction of point mutations anddominant lethal mutations in mice publication-title: Mol. Gen. Genet. – year: 1971 ident: BIB7 article-title: Evaluation and testing of drugs for mutagenicity: principles and problems publication-title: Technical Report Series No. 482 – volume: 130 start-page: 831 year: 1969 end-page: 834 ident: BIB1 article-title: A host-mediated microbial assay for the detection of mutagenic compounds publication-title: Proc. Soc. Exp. Biol. Med. – volume: 104 start-page: 27 year: 1969 end-page: 39 ident: BIB2 article-title: Formation of prototrophs in mixture of two auxotrophic mutants of publication-title: Mol. Gen. Genet. – year: 1970 ident: BIB3 article-title: The host-mediated assay, a practical procedure for evaluating potential mutagenic agents publication-title: Chemical Mutagenesis in Mammals and Man – volume: 18 start-page: 301 year: 1973 end-page: 309 ident: BIB6 article-title: A study of triflupromazine in dominant-lethal, cytogenetic and host-mediated assay publication-title: Mutation Res. – volume: 28 start-page: 129 year: 1971 ident: 10.1016/0165-1161(75)90002-3_BIB4 article-title: The influence of metabolism on mutagenic activity in the host-mediated assay publication-title: Arch. Toxikol. doi: 10.1007/BF00332388 – volume: 104 start-page: 27 year: 1969 ident: 10.1016/0165-1161(75)90002-3_BIB2 article-title: Formation of prototrophs in mixture of two auxotrophic mutants of Serratia marcescens HY by a transducing bacterial phage produced by some auxotrophs publication-title: Mol. Gen. Genet. doi: 10.1007/BF00277360 – volume: 117 start-page: 197 year: 1972 ident: 10.1016/0165-1161(75)90002-3_BIB5 article-title: Comparative investigations on the chemical induction of point mutations anddominant lethal mutations in mice publication-title: Mol. Gen. Genet. doi: 10.1007/BF00271647 – year: 1971 ident: 10.1016/0165-1161(75)90002-3_BIB7 article-title: Evaluation and testing of drugs for mutagenicity: principles and problems – volume: 130 start-page: 831 year: 1969 ident: 10.1016/0165-1161(75)90002-3_BIB1 article-title: A host-mediated microbial assay for the detection of mutagenic compounds – year: 1970 ident: 10.1016/0165-1161(75)90002-3_BIB3 article-title: The host-mediated assay, a practical procedure for evaluating potential mutagenic agents – volume: 18 start-page: 301 year: 1973 ident: 10.1016/0165-1161(75)90002-3_BIB6 article-title: A study of triflupromazine in dominant-lethal, cytogenetic and host-mediated assay publication-title: Mutation Res. doi: 10.1016/0027-5107(73)90214-5 |
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| SubjectTerms | Animals Genetics, Microbial Hydrazines - pharmacology Isoniazid - pharmacology Male Methods Mice Molecular Biology Mutagens - pharmacology Mutation Probability Salmonella typhimurium - drug effects Salmonella typhimurium - pathogenicity Serratia marcescens - drug effects Serratia marcescens - pathogenicity Time Factors |
| Title | Testing the mutagenic potency of chemical substances in a linear host-mediated assay I. Experimental microbiological basis |
| URI | https://dx.doi.org/10.1016/0165-1161(75)90002-3 https://www.ncbi.nlm.nih.gov/pubmed/1095922 |
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