Testing the mutagenic potency of chemical substances in a linear host-mediated assay I. Experimental microbiological basis

By the use of the mutagenic substance hydrazine sulphate it is shown that the currently used single determination method for determining point mutations in host-mediated assays with calculation of mutation frequencies can lead to erroneous results. The microbiological basis for a linear method is pr...

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Bibliographic Details
Published inMutation Research/Environmental Mutagenesis and Related Subjects Vol. 31; no. 4; pp. 205 - 216
Main Authors Grafe, Alfred, Lorenz, Rolf, Vollmar, Joachim
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.08.1975
Subjects
Animals
Genetics, Microbial
Hydrazines - pharmacology
Isoniazid - pharmacology
Male
Methods
Mice
Molecular Biology
Mutagens - pharmacology
Mutation
Probability
Salmonella typhimurium - drug effects
Salmonella typhimurium - pathogenicity
Serratia marcescens - drug effects
Serratia marcescens - pathogenicity
Time Factors
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ISSN0165-1161
0027-5107
DOI10.1016/0165-1161(75)90002-3

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Summary:By the use of the mutagenic substance hydrazine sulphate it is shown that the currently used single determination method for determining point mutations in host-mediated assays with calculation of mutation frequencies can lead to erroneous results. The microbiological basis for a linear method is presented in which the population growth of the auxotrophic and substance-induced mutants used in the test can be described mathematically during their logarithmic growth phase with regression lines.
ISSN:0165-1161
0027-5107
DOI:10.1016/0165-1161(75)90002-3