Acuticoccus kalidii sp. nov., a 1-aminocyclopropane-1-carboxylate (ACC) deaminase-producing endophyte from a root of Kalidium cuspidatum

• Published in: International journal of systematic and evolutionary microbiology Vol. 72; no. 10
• Main Authors: Tian, Jing, Xu, Lian, Zhang, Xu, Sun, Ji-Quan
• Format: Journal Article
• Language: English
• Published: 01.01.2022
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.005521

A 1-aminocyclopropane-1-carboxylate (ACC) deaminase-producing, Gram-stain-negative, strictly aerobic, non-motile, yellow-reddish, oval-shaped bacterial strain, designated M5D2P5 T , was isolated from a root of Kalidium cuspidatum , in Tumd Right Banner, Inner Mongolia, PR China. M5D2P5 T grew at 10–40 °C (optimum 30–35 °C), pH 5.0–10.0 (optimum pH 8.0) and with 0–7% NaCl (optimum 3.0 %). The strain was positive for catalase and oxidase. The phylogenetic trees based on 16S rRNA gene sequences indicated that M5D2P5 T clustered with Acuticoccus yangtzensis JL1095 T , and shared 98.0, 97.3, 97.2, 96.9 and less than 96.9 % 16S rRNA gene similarities to A. yangtzensis JL1095 T , Acuticoccus mangrovi B2012 T , Acuticoccus sediminis PTG4-2 T , Acuticoccus kandeliae J103 T , and all the other type strains, respectively. However, the phylogenomic tree showed it clustered with A. kandeliae J103 T . M5D2P5 T contained Q-10 as the major respiratory quinone, as well as two minor respiratory quinones, Q-7 and Q-8. Its major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified phospholipid, an unidentified glycolipid, and four unidentified lipids. The genomic DNA G+C content was 66.5 %. The digital DNA–DNA hybridization score and the average nucleotide identity based on blast values of M5D2P5 T to A. yangtzensis JL1095 T , A. kandeliae J103 T , A. mangrovi B2012 T , and A. sediminis PTG4-2 T , were 20.8, 23.7, 20.7, and 21.5 %, and 73.3, 79.5, 74.4, and 73.7 %, respectively. The phylogenetic and phenotypic characteristics allowed the discrimination of M5D2P5 T from its phylogenetic relatives. The novel species Acuticoccus kalidii sp. nov. is therefore proposed, and the type strain is M5D2P5 T (=CGMCC 1.19149 T =KCTC 92132 T ).