EFFICIENT FILTRATION OF VARIOUS VIRUSES THROUGH TWO TYPES OF MEMBRANE FILTERS

• Published in: Uirusu Vol. 25; no. 4; pp. 242 - 246
• Main Authors: SHINKAI, Kenkichi, NISHIMURA, Takuzo
• Format: Journal Article
• Language: Japanese
• Published: Japan The Japanese Society for Virology 1975
• Subjects: Filtration - instrumentation; Orthomyxoviridae; Simplexvirus; Vesicular stomatitis Indiana virus
• ISSN: 0042-6857; 1884-3433
• DOI: 10.2222/jsv.25.242

The efficiency of filtration of various viruses was compared between cellulose acetate membrane (CAM) and cellulose nitrate membrane (CNM). In addition, studies were made on the effects of virus diluents and pretreatment of these membrane filters on the filtration of various viruses. The results obtained are summarized as follows. (1) Herpes simplex virus (HSV), vesicular stomatitis virus (VSV), and influenza virus diluted in phosphate-buffered saline (PBS) or Eagle's minimum essential medium (MEM) were adsorbed completely to untreated CNM. Therefore, no infectivity was detected in the filtrate of any virus. When HSV, VSV and influenza virus diluted in the above-mentioned diluents were filtered through untreated 0.45μ CAM, the viral infectivity recovered in the resulting filtrate was about 45, 75, and 75%, respectively. (2) When HSV, VSV and influenza virus were diluted in PBS containing 0.1% egg-yolk or in MEM supplemented with 2% calf serum and filtered through untreated CAM or CNM, there was an increase in the amount of recovery of these viruses. (3) When CAM and CNM were pretreated with 1.3 to 5% calf serum or 0.25 to 1% polyvinylpyrrolidon (PVP), they were prevented from adsorbing HSV, VSV and influenza virus, and the recovery of viral infectivity was increased in the filtrate through each membrane.