Modulatory effects of interferon-γ on the fibronectin receptor function of squamous cell carcinoma cells in vitro
We previously showed that the in vivo invasion of a squamous cell carcinoma induced by the intradermal injection of tumor cells was significantly delayed after the IFN-γ-producing gene transfer to tumor cells. With respect to the mechanism of the delayed invasion, it was suggested that the IFN-γ mig...
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Published in | Journal of dermatological science Vol. 2; no. 6; pp. 422 - 427 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier Ireland Ltd
01.12.1991
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Subjects | |
Online Access | Get full text |
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Summary: | We previously showed that the in vivo invasion of a squamous cell carcinoma induced by the intradermal injection of tumor cells was significantly delayed after the IFN-γ-producing gene transfer to tumor cells. With respect to the mechanism of the delayed invasion, it was suggested that the IFN-γ might inhibit the adhesion of the cells to extracellular matrices (ECM) and the subsequent locomotion. Thus, we examined the effect of IFN-γ on the adhesion of Pam-T cells to ECM. The attachment of Pam-T cells to fibronectin (FN) was significantly higher than that to laminin (LN), collagen type I (COL I) or collagen type IV (COL IV) substrata. The attachment of FN was significantly enhanced specifically by the IFN-γ-treatment of the cells, although the attachment to LN, COL I or COL IV was not altered by IFN-γ. Neither IFN-α nor IFN-β had any effect on the attachment of Pam-T cells to FN. When Pam-T cells were treated with IFN-γ together with a neutralizable anti-IFN-γ antibody, this enhancement was completely abolished. Moreover, the attachment of IFN-γ-treated Pam-T cells as well as non-treated cells to FN was blocked by the synthetic peptide Arg-Gly-Asp-Ser (RGDS), but not by the control peptide Arg-Gly-Glu-Ser. Based on these results, we conclude that IFN-γ specifically enhances the adhesiveness of Pam-T cells to FN substrata by the modulation of integrin activity. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0923-1811 1873-569X |
DOI: | 10.1016/0923-1811(91)90006-J |