Modulatory effects of interferon-γ on the fibronectin receptor function of squamous cell carcinoma cells in vitro

• Published in: Journal of dermatological science Vol. 2; no. 6; pp. 422 - 427
• Main Authors: Maruguchi, Yukiya, Toda, Ken-Ichi, Watanabe, Yoshihiko, Imamura, Sadao
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ireland Ltd 01.12.1991
• Subjects: Adhesion; Amino Acid Sequence; Animals; Carcinoma, Squamous Cell - physiopathology; Carcinoma, Squamous Cell - therapy; Cell Adhesion - drug effects; Delayed invasion; Integrin activity; Interferon-gamma - pharmacology; Interferon-γ; Mice; Molecular Sequence Data; Oligopeptides - chemistry; Oligopeptides - pharmacology; Receptors, Fibronectin; Receptors, Immunologic - drug effects; Receptors, Immunologic - physiology; Squamous cell carcinoma; Tumor Cells, Cultured - drug effects; Tumor Cells, Cultured - physiology; Squamous cell carcinoma; Integrin activity; Delayed invasion; Interferon-γ; Adhesion
• ISSN: 0923-1811; 1873-569X
• DOI: 10.1016/0923-1811(91)90006-J

We previously showed that the in vivo invasion of a squamous cell carcinoma induced by the intradermal injection of tumor cells was significantly delayed after the IFN-γ-producing gene transfer to tumor cells. With respect to the mechanism of the delayed invasion, it was suggested that the IFN-γ might inhibit the adhesion of the cells to extracellular matrices (ECM) and the subsequent locomotion. Thus, we examined the effect of IFN-γ on the adhesion of Pam-T cells to ECM. The attachment of Pam-T cells to fibronectin (FN) was significantly higher than that to laminin (LN), collagen type I (COL I) or collagen type IV (COL IV) substrata. The attachment of FN was significantly enhanced specifically by the IFN-γ-treatment of the cells, although the attachment to LN, COL I or COL IV was not altered by IFN-γ. Neither IFN-α nor IFN-β had any effect on the attachment of Pam-T cells to FN. When Pam-T cells were treated with IFN-γ together with a neutralizable anti-IFN-γ antibody, this enhancement was completely abolished. Moreover, the attachment of IFN-γ-treated Pam-T cells as well as non-treated cells to FN was blocked by the synthetic peptide Arg-Gly-Asp-Ser (RGDS), but not by the control peptide Arg-Gly-Glu-Ser. Based on these results, we conclude that IFN-γ specifically enhances the adhesiveness of Pam-T cells to FN substrata by the modulation of integrin activity.