Diagnosis of tumor-like polypoid lesions of gallbladder by serum proteomic fingerprint

• Published in: Chinese journal of cancer research Vol. 20; no. 4; pp. 286 - 289
• Main Authors: Ge, Chun-lin, Luan, Feng-ming, Ma, Ning, Li, Yong-zhe, Hu, Chao-jun, Li, Ning
• Format: Journal Article
• Language: English
• Published: Heidelberg Chinese Anti-Cancer Association 01.12.2008; Department of General Surgery,First Affiliated Hospital,China Medical University,Shenyang 110001,China%Department of Clinical Laboratory,Peking Union Medical College Hospital,Peking Union Medical College & Chinese Academy of Medical Sciences,Beijing 100730,China
• Subjects: Medicine; Medicine & Public Health; Surface enhanced laser desorption/ionization time of flight mass spectrometry; Specific proteins; Diagnosis; Gallbladder disease; R735.8
• ISSN: 1000-9604; 1993-0631
• DOI: 10.1007/s11670-008-0286-7

Objective To search the specific serum proteins in tumor-like polypoid lesions of the gallbladder(PLG) patients. Methods Surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) technique and WCX Magnetic Beads were used to detect the serum proteomic fingerprint of 23 patients with tumor-like PLG, 21 patients with non tumor-like PLG and 26 normal persons. Biomarker Wizard and Biomarker Patterns Software were used in combination to analyze the data. Results In the preliminary screening, 22 representative specific proteins for tumor-like PLG were found. Seven specific proteins showed increased expression and 15 specific proteins showed decreased expression in the tumor-like PLG patients. Three specific proteins are selected for the diagnosis of the tumor-like PLG. Conclusion SELDI-TOF-MS technique can be used to select specific proteins for tumor-like PLG patients, which may be useful for the diagnosis of the tumor-like PLG and the differential diagnosis with the non tumor-like PLG.