In vivo inflammatory stimulation induces a transient change in the binding of thrombin to rat peritoneal macrophages

• Published in: Experimental cell research Vol. 201; no. 1; pp. 145 - 153
• Main Authors: Fisker, Sanne, Kudahl, Kirsten, Sonne, Ole
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.1992
• Subjects: Animals; Cells, Cultured; Inflammation - chemically induced; Inflammation - metabolism; Lipopolysaccharides - toxicity; Macrophage Activation; Macrophages - drug effects; Macrophages - metabolism; Male; Peritoneal Cavity - cytology; Protein Binding - drug effects; Rats; Rats, Inbred Strains; Receptors, Cell Surface - drug effects; Receptors, Thrombin; Thioglycolates - pharmacology; Thrombin - metabolism
• ISSN: 0014-4827; 1090-2422
• DOI: 10.1016/0014-4827(92)90358-F

The binding of 125I-labeled thrombin to rat peritoneal macrophages isolated 20 h after the ip injection of thioglycollate broth or lipopolysaccharide decreased to 20% of the value found in resident macrophages due to a decrease in the number of receptors. The binding returned to normal values within a week after the injection. The decline parallelled more or less the V max for the 5′-nucleotidase activity. This decrease in the binding of thrombin could not be explained by an immigration of monocytes into the peritoneal cavity, since the binding of 125I-labeled α 2-macroglobulin-trypsin complex increased 4.5-fold in the same cell population due to an increase in the number of receptors, and blood monocytes do not bind α 2-macroglobulin-trypsin complex. The increase in the binding of α 2-macroglobulinprotease complex parallelled an increase in the incorporation of glucosamine, although the latter did not increase to the same extent. Engulfment of plasma membrane after phagocytosis did not result in a decreased binding of thrombin, but preincubation at 37 °C with concanavalin A caused a minor reduction in the binding. There was a positive correlation between the binding of α 2-macroglobulin-trypsin complex and the fraction of polymorphonuclear leukocytes in the peritoneal exudate and a negative correlation between the binding of thrombin and the fraction of polymorphonuclear leukocytes in the exudate, when the inflammation was induced by a milder stimulus, sterile NaCl, indicating a common signal for the polymorphonuclear leukocyte chemotaxis and the macrophage differentiation.