Single nucleotide polymorphism analysis of 3′UTR rs713041 of Glutathione peroxidase 4 association with susceptibility to oral premalignant disorders

• Published in: Oral and maxillofacial surgery Vol. 29; no. 1; p. 89
• Main Authors: Subbiah, Usha, Sidhic, Nihala
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 21.04.2025; Springer Nature B.V
• Subjects: Genotype & phenotype; Medicine; Medicine & Public Health; Oral and Maxillofacial Surgery; Polymorphism; Prevalence; Single nucleotide polymorphism; Oral premalignancy disorder; Cancer
• ISSN: 1865-1569; 1865-1550; 1865-1569
• DOI: 10.1007/s10006-025-01380-0

Purpose Oral potentially malignant disorders (OPMDs) affect the oral mucosa and increase the risk of oral cancer. Glutathione peroxidase 4 ( GPX4 ), a key antioxidant mediator and regulator of ferroptosis, has garnered significant attention in cancer research. This study is to investigate the association between the single nucleotide polymorphism GPX4 3’UTR rs713041 and oral premalignancy disorders. Methods The rs713041of GPX4 were analysed using PCR-RFLP IN 600 subjects including OSMF, leukoplakia and healthy controls along with their habitual factors. Results Chewing and smoking habits were present in 52% and 69% of OSMF cases, and 62% and 57% of leukoplakia cases, respectively. Disease prevalence was 78% in males and 59% in females for OSMF, and 22% in males and 26% in females for leukoplakia. The allele frequency distribution for OSMF did not significantly deviate from Hardy-Weinberg equilibrium. The heterozygous TC genotype in OSMF showed a significant association with an odds ratio of 2.42 (CI: 1.58–3.72, P  = 0.00) compared to controls. Conclusion The GPX4 3’UTR T/C carrier genotype is associated with an increased risk of OSMF and leukoplakia. This genotype could serve as a predictive marker for the risk of oral premalignancy disorders. Clinical trail number Not applicable.