A technique for microinjection of peripheral nerve

Microinjection of peripheral nerves has proved especially useful in experimental studies in demyelination and neurotoxicology. A technique is described here for inducing a pure circumscribed demyelinating lesion in a peripheral nerve. Lysophosphatidyl choline (2 μl of 1%) is injected through a glass...

Full description

Saved in:
Bibliographic Details
Published inJournal of the neurological sciences Vol. 54; no. 2; pp. 317 - 323
Main Authors Ghabriel, M.N., Allt, G.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.05.1982
Subjects
Animals
Axons - drug effects
Dose-Response Relationship, Drug
Lysophosphatidylcholines - toxicity
Microinjections - instrumentation
Myelin Sheath - drug effects
Nerve Fibers, Myelinated - drug effects
Rats
Schwann Cells - drug effects
Sural Nerve - drug effects
Online AccessGet full text

Cover

More Information
Summary:Microinjection of peripheral nerves has proved especially useful in experimental studies in demyelination and neurotoxicology. A technique is described here for inducing a pure circumscribed demyelinating lesion in a peripheral nerve. Lysophosphatidyl choline (2 μl of 1%) is injected through a glass micropipette (3–10 μm tip) with the aid of a micromanipulator.
ISSN:0022-510X
1878-5883
DOI:10.1016/0022-510X(82)90192-7