Probiotics and prebiotics alleviate behavioral deficits, inflammatory response, and gut dysbiosis in prenatal VPA-induced rodent model of autism

• Published in: Physiology & behavior Vol. 256; p. 113961
• Main Authors: Adıgüzel, Emre, Çiçek, Betül, Ünal, Gökhan, Aydın, Mehmet Fatih, Barlak-Keti, Didem
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.11.2022
• Subjects: Autism; Behavioral deficits; Gut-brain axis; Microbiota; Social interaction; Autism; Microbiota; Behavioral deficits; Social interaction; Gut-brain axis
• ISSN: 0031-9384; 1873-507X
• DOI: 10.1016/j.physbeh.2022.113961

•Prenatal VPA exposure caused behavioral deficits, inflammatory response, and gut dysbiosis.•The probiotic and prebiotic treatments alleviated VPA-induced sociability and social preference deficits.•The treatments also presented positive outcomes in terms of anxiety and repetitive behaviors.•Probiotic and combined (probiotic+prebiotic) treatments increased serum IL-10 levels and decreased serum IL-6 levels. Autism spectrum disorders are neuropsychiatric conditions characterized by social interaction and communication disorders and repetitive stereotypical behaviors. These disorders are also accompanied by an inflammatory status. Bidirectional communication between microbiome, gut, and brain has been discovered as a major mechanism influencing core symptoms and biomarkers of autism. Therefore, the modulation of the gut microbiota in autism has recently attracted interest. In this study, probiotic- and prebiotic-mediated modulation of the gut microbiota was compared in terms of different symptoms and findings in an experimental autism model. Valproic acid (VPA) (500 mg/kg) was administered to Wistar rats (on prenatal day 12.5) to induce autistic-like behaviors. Based on the supply of probiotics and prebiotics, animals were grouped as control (saline), autistic-like (prenatal VPA), probiotic (prenatal VPA + 22.5 × 109 cfu/day probiotic), prebiotic (prenatal VPA + 100 mg/day prebiotic), and combined treatment (prenatal VPA + 22.5 × 109 cfu/day probiotic + 100 mg/day prebiotic). After the treatment process, behavioral tests (social behaviors, anxiety, stereotypical behavior, sensorimotor gating, and behavioral despair) and biochemical analyses (serum and brain tissue) were conducted, and the quantities of some phyla and genera were determined in stool samples. Significant positive effects of probiotic and combined treatments were observed on the sociability, social interaction, and anxiety parameters. In addition, all three treatments had positive effects on stereotypical behavior. However, the treatments did not affect sensorimotor gating deficits and behavioral despair. Further, probiotic treatment reversed the VPA-induced increase and decrease in serum IL-6 and IL-10 levels, respectively. Combined treatment also significantly increased the IL-10 levels. Prenatal VPA exposure decreased 5-hydroxytryptamine (5-HT) levels in the prefrontal cortex of the brain; however, combined treatment reversed this decrease. Prenatal VPA exposure also caused a decrease in Bacteroidetes/Firmicutes ratio in the gut microbiota, while the probiotic treatment significantly increased this ratio. These findings indicate that probiotic- and prebiotic-mediated microbial modulation may represent a new therapeutic approach to alleviate autistic-like symptoms. [Display omitted]