Shotgun Lipidomics of Rodent Tissues

Lipids play a vital role as essential components of all prokaryotic and eukaryotic cells. Constant technological improvements in mass spectrometry have made lipidomics a powerful analytical tool for monitoring tissue lipidome compositions in homeostatic as well as disease states. This paper presents...

Full description

Saved in:
Bibliographic Details
Published inJournal of visualized experiments no. 189
Main Authors Lavrynenko, Oksana, Dijon, Sophie, Titz, Björn, Ivanov, Nikolai V
Format Journal Article
LanguageEnglish
Published United States 18.11.2022
Subjects
Animals
Eukaryotic Cells
Firearms
Lipidomics
Lipids
Mice
Rodentia
Online AccessGet more information

Cover

More Information
Summary:Lipids play a vital role as essential components of all prokaryotic and eukaryotic cells. Constant technological improvements in mass spectrometry have made lipidomics a powerful analytical tool for monitoring tissue lipidome compositions in homeostatic as well as disease states. This paper presents a step-by-step protocol for a shotgun lipid analysis method that supports the simultaneous detection and quantification of a few hundred molecular lipid species in different tissue and biofluid samples at high throughput. This method leverages automated nano-flow direct injection of a total lipid extract spiked with labeled internal standards without chromatographic separation into a high-resolution mass spectrometry instrument. Starting from sub-microgram amounts of rodent tissue, the MS analysis takes 10 min per sample and covers up to 400 lipids from 14 lipid classes in mouse lung tissue. The method presented here is well suited for studying disease mechanisms and identifying and quantifying biomarkers that indicate early toxicity or beneficial effects within rodent tissues.
ISSN:1940-087X
DOI:10.3791/63726