Effect of glucose on beta-adrenergic induced downregulation of insulin receptor binding in human fat cells

The effect of beta-adrenergic stimulation on specific insulin binding to isolated human fat cells was investigated at 24°C and at 37°C. In the abscense of glucose isoprenaline caused a 40% decrease in high affinity insulin binding at both temperatures. At 37°C the reduction in binding was completely...

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Published inBiochemical and biophysical research communications Vol. 122; no. 1; pp. 97 - 102
Main Authors Arner, Peter, Hellmér, Johan, Ewerth, Staffan, Östman, Jan
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 18.07.1984
Subjects
Adipose Tissue - metabolism
Adult
Female
Glucose - pharmacology
Humans
In Vitro Techniques
Isoproterenol - pharmacology
Lipolysis - drug effects
Male
Middle Aged
Receptor, Insulin - drug effects
Receptor, Insulin - metabolism
Receptors, Adrenergic, beta - drug effects
Temperature
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Summary:The effect of beta-adrenergic stimulation on specific insulin binding to isolated human fat cells was investigated at 24°C and at 37°C. In the abscense of glucose isoprenaline caused a 40% decrease in high affinity insulin binding at both temperatures. At 37°C the reduction in binding was completely offset by the addition of glucose to the medium. A maximum effect of glucose occured at 5 mmol/l. At 24°C, however, there was no effect of glucose on insulin binding. The effects of glucose and isoprenaline on insulin binding were not related to the lipolytic activities these two agents. In conclusion, low amounts of glucose prevent catecholamine induced down-regulation of insulin receptor binding in human fat cells at physiological temperature.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(84)90444-3