PpGATA21 Enhances the Expression of PpGA2ox7 to Regulate the Mechanism of Cerasus humilis Rootstock-Mediated Dwarf in Peach Trees

• Published in: International journal of molecular sciences Vol. 25; no. 13; p. 7402
• Main Authors: Li, Xiuzhen, Wang, Ruxin, Wang, Yuman, Li, Xueqiang, Shi, Qiaofang, Yu, Yihe
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 05.07.2024
• Subjects: Biosynthesis; Cerasus humilis; dwarfing rootstock; Flowers & plants; Food quality; Fruits; GA 2-oxidase; Gene expression; Gene Expression Regulation, Plant; gibberellin; Gibberellins - metabolism; Indoleacetic Acids - metabolism; Influence; Metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Roots - genetics; Plant Roots - growth & development; Plant Roots - metabolism; PpGATA21; Promoter Regions, Genetic; Prunus persica - genetics; Prunus persica - growth & development; Prunus persica - metabolism; Signal transduction; Transcription factors; Trees; Trees - genetics; Trees - growth & development; Yeast; GA 2-oxidase; dwarfing rootstock; gibberellin; Cerasus humilis; PpGATA21
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25137402

Dwarfing rootstocks enhance planting density, lower tree height, and reduce both labor in peach production. is distinguished by its dwarf stature, rapid growth, and robust fruiting capabilities, presenting substantial potential for further development. In this study, Ruipan 4 was used as the scion and grafted onto and , respectively. The results indicate that compared to grafting combination R/M (Ruipan 4/ ), grafting combination R/O (Ruipan 4/ ) plants show a significant reduction in height and a significant increase in flower buds. RNA-seq indicates that genes related to gibberellin (GA) and auxin metabolism are involved in the dwarfing process of scions mediated by . The expression levels of the GA metabolism-related gene significantly increased in R/O and are strongly correlated with plant height, branch length, and internode length. Furthermore, GA levels were significantly reduced in R/O. The transcription factor PpGATA21 was identified through yeast one-hybrid screening of the promoter. Yeast one-hybrid (Y1H) and dual-luciferase reporter (DLR) demonstrate that PpGATA21 can bind to the promoter of and activate its expression. Overall, PpGATA21 activates the expression of the GA-related gene , resulting in reduced GA levels and consequent dwarfing of plants mediated by This study provides new insights into the mechanisms of and offers a scientific foundation for the dwarfing and high-density cultivation of peach trees.