Histochemical analysis of stem and fiber of ramie (Boehmeria nivea (L.) Gaud var. Murakami)

Analyzes of samples of stem and fiber Boehmeria nivea var. Murakami were performed to increase information on the plant and disseminate its multiple possibilities of use. The histochemical analyzes with dyes and reagents showed the presence of lignified, suberous and cutinized cell walls with Safran...

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Published inActa scientiarum. Biological sciences Vol. 40; no. 1; p. 37207
Main Authors Marinho, Nelson Potenciano, Muñiz, Graciela Ines Bolzon de, Nisgoski, Silvana, Venson, Ivan, Cademartori, Pedro Henrique Gonzales de, Andrade, Alan Sulato de
Format Journal Article
LanguageEnglish
Published Maringa Editora da Universidade Estadual de Maringá - EDUEM 01.04.2018
Universidade Estadual de Maringá
Subjects
Ashes
Bark
Boehmeria nivea
Cell walls
Cellulose
chemical composition
Ethanol
Hemicellulose
Lignin
Methylene blue
morphological structure
Mucilage
Pectin
ramie
Sodium hydroxide
Toluene
Xylem
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Summary:Analyzes of samples of stem and fiber Boehmeria nivea var. Murakami were performed to increase information on the plant and disseminate its multiple possibilities of use. The histochemical analyzes with dyes and reagents showed the presence of lignified, suberous and cutinized cell walls with Safranin in the xylem region. The presence of pectin-cellulose with Astra Blue was confirmed in the regions of fiber bundles (cortex). In all regions of the stem were identified substances such as fat and mucilage with Methylene Blue evidencing high concentrations in the regions of fiber bundles and bark (violet blue). By means of Lugol (dark brown) starches were identified in several parts of the stem with concentrations in the xylem and epidermal regions. Through analyzes by EDS it was possible to identify F, Mg, Al, Si, K and Ca with predominance of K. In the chemical analyzes of the fibers were found 71.75 of cellulose, 12.11 of hemicelluloses, 1.06 of lignin and 1.70% of ashes. The percentages of extractives soluble in hot and cold water were 5.28 and 3.12% respectively, for Ethanol-Toluene was 8.55 and NaOH (1%) 27.27%. Histochemical analyzes revealed important characteristics of the fiber and stem contributing with better knowledge of the species.
ISSN:1679-9283
1807-863X
DOI:10.4025/actascibiolsci.v40i1.37207