Enhancement of the binding of C1q to immune complexes by polyethylene glycol

The binding of 125I-labelled human C1q to insoluble rabbit IgG:ovalbumin immune complexes was enhanced by polyethylene glycol (PEG, Mr 8 x 10(3)) in the concn range 0-2.5% (w/v). C1q with native immunoglobulin bindings sites rendered inactive by diethylpyrocarbonate treatment did not bind to immune...

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Bibliographic Details
Published inMolecular immunology Vol. 25; no. 3; p. 263
Main Authors Wines, B D, Easterbrook-Smith, S B
Format Journal Article
LanguageEnglish
Published England 01.03.1988
Subjects
Animals
Antigen-Antibody Complex - immunology
Chemical Phenomena
Chemistry, Physical
Complement Activating Enzymes - immunology
Complement C1 - immunology
Complement C1q
Dose-Response Relationship, Immunologic
Humans
Immunoglobulin G - immunology
Ovalbumin - immunology
Polyethylene Glycols - pharmacology
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Summary:The binding of 125I-labelled human C1q to insoluble rabbit IgG:ovalbumin immune complexes was enhanced by polyethylene glycol (PEG, Mr 8 x 10(3)) in the concn range 0-2.5% (w/v). C1q with native immunoglobulin bindings sites rendered inactive by diethylpyrocarbonate treatment did not bind to immune complexes in the presence of PEG. The ionic strength dependence of the binding was independent of the presence of PEG. There was a linear relationship between the logarithm of the apparent affinity constant of the C1q:immune complex interaction and PEG concn.
ISSN:0161-5890
DOI:10.1016/0161-5890(88)90017-X