A rapid method for assessment of a macrophage chemotactant produced by SaD2 fibrosarcoma cells in vitro

A new rapid method for assessing murine macrophage chemotaxis was developed using peritoneal exudate cells labeled with [5,6-3H]uridine in modified Boyden chambers with double polycarbonate filters. The method gave positive results with endotoxin-treated mouse serum and with serum-free culture super...

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Bibliographic Details
Published inJournal of immunological methods Vol. 32; no. 2; p. 195
Main Authors Simon, L S, Patterson, R, Jones, T L
Format Journal Article
LanguageEnglish
Published Netherlands 1980
Subjects
Animals
Chemotactic Factors - analysis
Fibrosarcoma - immunology
Fibrosarcoma - metabolism
Macrophages - physiology
Male
Mice
Neoplasms, Experimental - immunology
Radiochemistry - methods
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Summary:A new rapid method for assessing murine macrophage chemotaxis was developed using peritoneal exudate cells labeled with [5,6-3H]uridine in modified Boyden chambers with double polycarbonate filters. The method gave positive results with endotoxin-treated mouse serum and with serum-free culture supernatant of the DBA/2 SaD2 fibrosarcoma. These results correlated with results obtained with conventional microscopic assessment of chemotaxis. Using this method, the SaD2 supernatant was shown to be chemotactic rather than chemokinetic.
ISSN:0022-1759
DOI:10.1016/0022-1759(80)90073-3