Reduced levels of mRNA for myofibrillar proteins in skeletal muscle from septic rats

The influence of sepsis on transcription of myofibrillar proteins in skeletal muscle was studied in rats. Sepsis was induced by cecal ligation and puncture (CLP); control rats were sham-operated. Sixteen hours later, muscle levels of mRNA for myofibrillar proteins were determined by using cDNA probe...

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Published inLife sciences (1973) Vol. 49; no. 10; p. 753
Main Authors Hasselgren, P O, Tricoli, J V, Wieczorek, D, Steigerwald, K A, Angerås, U, Hall-Angerås, M, Fischer, J E
Format Journal Article
LanguageEnglish
Published Netherlands 1991
Subjects
Actins - genetics
Animals
Bacterial Infections - genetics
Blotting, Northern
DNA - genetics
DNA Probes
Male
Muscle Proteins - biosynthesis
Myosins - genetics
Nucleic Acid Hybridization
Rats
Rats, Inbred Strains
RNA, Messenger - analysis
RNA, Messenger - genetics
Transcription, Genetic
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Summary:The influence of sepsis on transcription of myofibrillar proteins in skeletal muscle was studied in rats. Sepsis was induced by cecal ligation and puncture (CLP); control rats were sham-operated. Sixteen hours later, muscle levels of mRNA for myofibrillar proteins were determined by using cDNA probes specific for transcripts for alpha actin and myosin heavy chain. Sepsis resulted in a 2-6 fold decrease in alpha actin mRNA levels and an even more pronounced reduction in myosin heavy chain mRNA levels. Results suggest that sepsis-induced reduction of muscle protein synthesis is at least partly regulated at the transcriptional level.
ISSN:0024-3205
DOI:10.1016/0024-3205(91)90108-N