A Novel Protocol Permitting the Use of Frozen Cell Cultures on Low Earth Orbit

Cell culture on orbit is complicated by numerous operational constraints, including g-loads on the ascent, vibrations, transit time to International Space Station, and delays in experiment initiation. Cryopreserving cells before launch would negate these factors. However, defrosting these cells is p...

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Bibliographic Details
Published inGravitational and space research Vol. 8; no. 1; pp. 25 - 30
Main Authors Kidder, L. S., Zea, L., Countryman, SM, Stodieck, L. S., Hammer, B. E.
Format Journal Article
LanguageEnglish
Published Sciendo 01.05.2020
Subjects
Cell culture on ISS
Cryopreserved cells
Defrost frozen cultures on orbit
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Summary:Cell culture on orbit is complicated by numerous operational constraints, including g-loads on the ascent, vibrations, transit time to International Space Station, and delays in experiment initiation. Cryopreserving cells before launch would negate these factors. However, defrosting these cells is problematic, since the traditional method of employing a water bath is not possible. We here describe a unique apparatus designed to accomplish this in a microgravitational environment. This apparatus resulted in rapid defrost of cryopreserved cell cultures and allowed successful tissue culture operations on the station for periods of up to 21 days.
ISSN:2332-7774
2332-7774
DOI:10.2478/gsr-2020-0003