Structural and serological characterization of Hafnia alvei lipopolysaccharide core region

The structures and serological activities of core oligosaccharide of Hafnia alvei strains have been investigated. Methylation analysis, NMR spectroscopy and various specific degradation procedures were the principal methods used. It is concluded that, core hexasaccharides are identical in the lipopo...

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Published inActa biochimica polonica Vol. 42; no. 1; pp. 51 - 54
Main Authors Lugowski, C, Niedziela, T, Jachymek, W, Klonowska, A, Czarny, A, Rowiński, S, Petersson, C, Kenne, L
Format Journal Article
LanguageEnglish
Published Poland 1995
Subjects
Carbohydrate Sequence
Chromatography, Gel
Enterobacteriaceae - chemistry
Gas Chromatography-Mass Spectrometry
Glucose - chemistry
Heptoses - chemistry
Lipopolysaccharides - chemistry
Lipopolysaccharides - immunology
Magnetic Resonance Spectroscopy
Mannose - chemistry
Methylation
Molecular Sequence Data
Oxidation-Reduction
Tetanus Toxoid - immunology
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Summary:The structures and serological activities of core oligosaccharide of Hafnia alvei strains have been investigated. Methylation analysis, NMR spectroscopy and various specific degradation procedures were the principal methods used. It is concluded that, core hexasaccharides are identical in the lipopolysaccharides tested and are built of two glucose, three heptose and one 2-keto-3-deoxyoctulosonic acid residues. The antiserum raised against the ATCC13337 oligosaccharide core-tetanus toxoid conjugate cross-reacted strongly with all lipopolysaccharides used as antigens in ELISA test, suggesting that this core region is the common structure in the Hafnia genus.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0001-527X
1734-154X
DOI:10.18388/abp.1995_4667