Response to Hepatocarcinoma Hca-F of Mice Immunized with Heat Shock Protein 70 from Elemene Combo Tumor Cell Vaccine

• Published in: Cellular & molecular immunology Vol. 3; no. 4; pp. 291 - 295
• Main Authors: Guo, Lianying, Shi, Guangxia, Gao, Zhihong, Shen, Jie, Xing, Rong, Qian, Zhenchao
• Format: Journal Article
• Language: English
• Published: China Institute for Cancer Biotherapy, Dalian Medical University, Dalian 116027,China 01.08.2006
• Subjects: Animals; Cancer Vaccines - administration & dosage; Cancer Vaccines - immunology; Carcinoma, Hepatocellular - immunology; Carcinoma, Hepatocellular - prevention & control; Carcinoma, Hepatocellular - therapy; Cell Cycle - drug effects; HSC70 Heat-Shock Proteins - administration & dosage; HSC70 Heat-Shock Proteins - immunology; Immunization; Liver Neoplasms - immunology; Liver Neoplasms - prevention & control; Liver Neoplasms - therapy; Mice; Mice, Inbred BALB C; Multiprotein Complexes - administration & dosage; Multiprotein Complexes - immunology; Neoplasm Transplantation; Spleen - cytology; Spleen - drug effects; Spleen - immunology; Survival Rate; 榄香烯; 热休克蛋白; Hca-F; HSP70; EC-TCV; immune response
• ISSN: 1672-7681; 2042-0226

To analyze immune response to murine hepatocarcinoma Hca-F of mice immunized with heat shock protein 70 （HSP70） derived from elemene combo tumor cell vaccine （EC-TCV） of Hca-F, HSP70 was isolated from EC-TCV by ADP affinity chromatography. Mice were immunized with HSP70 intraperitoneally three times and spleen calls were sampled. For cells, their proliferation and cytotoxicity against Hca-F were measured with MTT assay and their phenotypes were analyzed with flow cytometry. Spleen cells of immunized mice with HSP70 exhibited more potent cytotoxicity against Hca-F and proliferation than that of normal control mice, but less potent than that of mice immunized with EC-TCV. Among three groups, the percent of T6 T lymphocytes in the mice immunized with HSP70 （35.5%） was the highest compared with 6.25% in normal mice, and 28.4% in the mice immunized with EC-TCV. Immunization of HSP70 derived from EC-TCV could elicit potent immune response to Hca-F. HSP70 is one of elements inducing anti-tumor immune responses against Hca-F.