Molecular analysis and mapping of two genes encoding maize glutathione S-transferases (GST 1 and GST 2)

• Published in: Molecular & general genetics Vol. 248; no. 5; pp. 535 - 539
• Main Authors: Rossini, L, Pe, M.E, Frova, C, Hein, K, Sari-Gorla, M. (Milan Univ. (Italy). Dept. of Genetics and Microbiology)
• Format: Journal Article
• Language: English
• Published: Germany 01.01.1995
• Subjects: Alleles; allelism; CARTE GENETIQUE; Chromosome Mapping; chromosomes; EXPRESION GENICA; EXPRESSION DES GENES; GENE; Gene Expression Regulation, Plant; gene location; GENES; genetic markers; glutathione transferase; Glutathione Transferase - genetics; gsti gene; gstii gene; Isoenzymes - genetics; linkage (genetics); loci; MAPAS GENETICOS; messenger RNA; mutation; PEPTIDE; PEPTIDOS; restriction fragment length polymorphism; structural genes; transcription (genetics); TRANSFERASAS; TRANSFERASE; ZEA MAYS; Zea mays - enzymology; Zea mays - genetics
• ISSN: 0026-8925; 1432-1874
• DOI: 10.1007/BF02423448

Maize glutathione S-transferase (GST) isozymes are encoded by a gene family comprising at least five genes, three of which (Gst 1, 2 and 3) have recently been isolated and sequenced. The enzymes are active as homo or heterodimers and exhibit intraspecific polymorphism including a "null" variant for the two major isoforms expressed in roots. Northern blot analyses performed on total root RNA from "null" and "plus" genotypes, using Gst 1- and Gst 2-specific probes, indicated that the Gst 1 gene controls the expression of the two major GST isoforms expressed in roots. Gst 1 and Gst 2 were mapped by RFLP analysis using an F2 population of 149 individuals previously characterized. Gst 1 was localized on the long arm of chromosome 8, while two putative Gst 2 loci were mapped to chromosomes 8 (70 cM from Gst 1) and 10, respectively.