Activation of a procarcinogen to a mutagen by cell-free extracts of anaerobic bacteria

• Published in: Mutation Research/Environmental Mutagenesis and Related Subjects Vol. 46; no. 4; pp. 261 - 264
• Main Authors: McCoy, Elena C., Speck, William T., Rosenkranz, Herbert S.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.1977
• Subjects: Aerobiosis; Anaerobiosis; Bacteroides fragilis - metabolism; Cell-Free System; Clostridium perfringens - metabolism; Fluorenes - metabolism; Mutagens - metabolism; Mutation; Species Specificity

The Salmonella mutagenicity assay can be coupled to cell-free preparations derived from anaerobic bacteria ( Clostridium perfringens and Bacteroides fragilis) to activate a procarcinogen to a mutagen. This activity is destroyed by heating and by digestion with pronase and it is sensitive to oxygen....