Activation of a procarcinogen to a mutagen by cell-free extracts of anaerobic bacteria

The Salmonella mutagenicity assay can be coupled to cell-free preparations derived from anaerobic bacteria ( Clostridium perfringens and Bacteroides fragilis) to activate a procarcinogen to a mutagen. This activity is destroyed by heating and by digestion with pronase and it is sensitive to oxygen....

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Bibliographic Details
Published inMutation Research/Environmental Mutagenesis and Related Subjects Vol. 46; no. 4; pp. 261 - 264
Main Authors McCoy, Elena C., Speck, William T., Rosenkranz, Herbert S.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.08.1977
Subjects
Aerobiosis
Anaerobiosis
Bacteroides fragilis - metabolism
Cell-Free System
Clostridium perfringens - metabolism
Fluorenes - metabolism
Mutagens - metabolism
Mutation
Species Specificity
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Summary:The Salmonella mutagenicity assay can be coupled to cell-free preparations derived from anaerobic bacteria ( Clostridium perfringens and Bacteroides fragilis) to activate a procarcinogen to a mutagen. This activity is destroyed by heating and by digestion with pronase and it is sensitive to oxygen. These findings indicate that the Salmonella mutagenicity assay can be adapted to the study of the role of anaerobes in the activation of carcinogens.
ISSN:0165-1161
0027-5107
DOI:10.1016/0165-1161(77)90002-4