Analytical and biological analyses of test materials from the synthetic fuel technologies. I. Mutagenicity of crude oils determined by the Salmonella typhimurium/microsomal activation system

• Published in: Mutation research Vol. 57; no. 3; p. 265
• Main Authors: Epler, J L, Young, J A, Hardigree, A A, Rao, T K, Guerin, M R, Rubin, I B, Ho, C H, Clark, B R
• Format: Journal Article
• Language: English
• Published: Netherlands 01.07.1978
• Subjects: Coal - toxicity; Environmental Pollutants - toxicity; Fossil Fuels - toxicity; Fuel Oils - toxicity; Genetic Techniques; Industrial Waste; Microsomes, Liver - metabolism; Mutagens; Salmonella typhimurium - genetics
• ISSN: 0027-5107
• DOI: 10.1016/0027-5107(78)90211-7

We have assayed the mutagenicity of crude industrial products and effluents with the Salmonella/microsomal activation system. Test materials (crude products from coal-conversion processes and natural crude oils) were initially fractionated into primary classes by liquid--liquid extraction and then further fractionated by column chromatography. Prescreening was accomplished over a wide concentration range with the Ames tester strains. Active fractions (mainly the neutral fractions containing polycyclic aromatic hydrocarbons and certain basic fractions) can be identified, and dose--response relationships can be established. Standard values are expressed as revertants/mg of the test material assayed with frameshift strain TA98 including metabolic activation with rat-liver preparations. Total mutagenic activity of synthetic fuel samples was consistently higher than that of natural crude "controls." Activities of subfractions are roughly additive and presumably reflect the mutagenic potential of the whole test material. These results are being extended to other genetic assays. Chemical identification is carried out along with the bioassays.