Differences between human lymphocyte subpopulations with respect to the uptake and incorporation of [ 3H]uridine and ribonucleoside triphosphate pools
Cellular [ 3H]uridine uptake and incorporation into RNA were compared in human tonsil lymphocyte subpopulations obtained by separation on nylon-wool column. While the nonadherent subpopulation, Fraction I, contained 74.5 ± 8% E-rosette forming T-lymphocytes, the adherent fraction, Fraction II, conta...
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Published in | Biochemical medicine Vol. 19; no. 2; pp. 218 - 230 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
01.04.1978
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Subjects | |
Online Access | Get full text |
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Summary: | Cellular [
3H]uridine uptake and incorporation into RNA were compared in human tonsil lymphocyte subpopulations obtained by separation on nylon-wool column. While the nonadherent subpopulation, Fraction I, contained 74.5 ± 8% E-rosette forming T-lymphocytes, the adherent fraction, Fraction II, contained 6.5 ± 3.5% of the same cells.
The B-cell-enriched Fraction II of lymphocytes, freshly prepared from tonsils, was 2.5-3 times as active as Fraction I with respect to both uptake and incorporation of [
3H]uridine. However, in phytohaemagglutinin-stimulated cultures the T-cell-enriched Fraction I proved to be more active with regard to
3H-uridine incorporation.
The size of the four ribonucleoside triphosphate pools determined by the RNA-polymerase test was practically the same in both lymphocyte subpopulations. The radioactivity of the acid soluble cell fraction after [
3H]uridine labeling consisted of about 60% [
3H]UTP in both B- and T-cell-rich lymphocyte fractions, as determined by chromatography on PEI cellulose.
On the basis of these results it seems unlikely that [
3H] uridine labeling is a specific marker of either of the lymphocyte subpopulations. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0006-2944 1557-7996 |
DOI: | 10.1016/0006-2944(78)90023-6 |