Isolation and characterization of recombinant DNA plasmids carrying Drosophila tRNA genes

Recombinant plasmids carrying Drosophila melanogaster tRNA genes were constructed by ligation of HindIII-cleaved Drosophila DNA to HindIII cut pBR322 DNA. 90 clones were isolated that contained genes for one or more of eleven tRNAs. 43 of the plasmids were characterized by a number of methods: restr...

Full description

Saved in:
Bibliographic Details
Published inGene Vol. 7; no. 3; pp. 197 - 215
Main Authors Dunn, Robert, Delaney, A.D., Gillam, I.C., Hayashi, S., Tener, G.M., Grigliatti, T., Misra, V., Spurr, M.G., Taylor, D.M., Miller, R.C.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.11.1979
Subjects
Online AccessGet full text

Cover

Loading…
More Information
Summary:Recombinant plasmids carrying Drosophila melanogaster tRNA genes were constructed by ligation of HindIII-cleaved Drosophila DNA to HindIII cut pBR322 DNA. 90 clones were isolated that contained genes for one or more of eleven tRNAs. 43 of the plasmids were characterized by a number of methods: restriction nuclease digestion; agarose gel electrophoresis; hybridization with individual, purified, 125I-labelled Drosophila tRNA molecules and in situ hybridization to Drosophila chromosomes. The results show that several different tRNA genes have been isolated which code for single, specific isoacceptors. The DNAs from 8 plasmids each hybridize to single sites on Drosophila polytene chromosomes. In addition, the data show examples of two different plasmids hybridizing to different loci coding for the same tRNA; this means that we have isolated representatives of tRNA genes which map at widely separated points on the Drosophila genome.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(79)90046-5