Suppression of in vitro immunoglobulin biosynthesis in bovine spleen cells by bovine viral diarrhea virus

• Published in: Clinical immunology and immunopathology Vol. 13; no. 3; pp. 254 - 260
• Main Authors: Atluru, Durgaprasadarao, Notowidjojo, Wardjiman, Johnson, Donald W., Muscoplat, Charles C.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.1979
• Subjects: Animals; Bovine Virus Diarrhea-Mucosal Disease - immunology; Cattle; Cattle Diseases - immunology; Cell Survival; Cells, Cultured; Cytoplasm - immunology; Diarrhea Viruses, Bovine Viral - immunology; Horses; Immunoglobulin G - biosynthesis; Immunoglobulin M - biosynthesis; Immunosuppression; Pokeweed Mitogens - pharmacology; RNA Viruses - immunology; Spleen - immunology; Virus Replication
• ISSN: 0090-1229; 1090-2341
• DOI: 10.1016/0090-1229(79)90070-9

Incubation of bovine splenic lymphoid cells with pokeweed mitogen (PWM) and bovine viral diarrhea (BVD) virus for 5 days caused a consistent, statistically significant decrease in plasma cell development (cells containing intracytoplasmic immunoglobulin) as well as IgG or IgM synthesis compared to cultures without BVD virus. Suppression of plasma cell development and immunoglobulin synthesis was not due to decreased cell viability in virus-infected cultures even though virus replication was evident in both PWM-stimulated as well as control cultures. Finally, it was shown that heat-inactivated BVD virus was not capable of suppressing either plasma cell development or immunoglobulin synthesis. Thus, these data support the hypothesis that BVD virus can alter B-cell responses, plasma cell development, and subsequent synthesis of IgG and IgM immunoglobulins.