Incorporation of [ 35S]sulphate into mucopolysaccharide by teleost cartilage in vitro: The influence of mammalian growth hormone, teleost plasma, and mammalian plasma

• Published in: General and comparative endocrinology Vol. 32; no. 2; pp. 187 - 194
• Main Author: Ash, Patricia J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.1977
• Subjects: Animals; Blood; Cartilage - drug effects; Cartilage - metabolism; Culture Media; Female; Fishes; Glycosaminoglycans - biosynthesis; Growth Hormone - pharmacology; Horses; Kinetics; Organ Culture Techniques; Species Specificity; Sulfates - metabolism; Sulfur Radioisotopes; Trout
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/0016-6480(77)90150-2

Branchial cartilages from two species of teleost fish, Salmo gairdneri and Tilapia andersoni, were found to be capable of synthesizing mucopolysaccharide for up to 4 days in vitro. Synthesis of mucopolysaccharide, the cartilage matrix, was measured by monitoring the amount of bound [ 35S]sulphate incorporated by the fish cartilage units. The binding of 35S appeared to be increased by the addition of bovine growth hormone to the organ culture medium. Plasma from Tilapia did not contain stimulatory somatomedin-like activity, when added to either Tilapia or rat cartilage cultures. However, mammalian serum and plasma, known to be rich in somatomedin, markedly stimulated the binding of 35S by Tilapia cartilage units in vitro. The degree of stimulation of 35S binding in fish cartilage by mammalian serum was greater and more consistent than that observed with bovine growth hormone. The possibility of a direct effect of growth hormone on skeletal growth in teleost fish is discussed.