Interaction of microtubules with the actin cytoskeleton via cross-talk of EB1-containing +TIPs and γ-actin in epithelial cells

• Published in: Oncotarget Vol. 7; no. 45; pp. 72699 - 72715
• Main Authors: Dugina, Vera, Alieva, Irina, Khromova, Natalya, Kireev, Igor, Gunning, Peter W, Kopnin, Pavel
• Format: Journal Article
• Language: English
• Published: United States Impact Journals LLC 08.11.2016
• Subjects: Actins - metabolism; Cell Line; Cytoplasm - metabolism; Cytoskeleton - metabolism; Epithelial Cells - metabolism; Humans; Microtubule-Associated Proteins - metabolism; Microtubules - chemistry; Microtubules - metabolism; Protein Binding; Protein Isoforms; Protein Multimerization; Protein Transport; Research Paper; γ-actin; actin isoforms; β-actin; microtubules; EB1; TIPs
• ISSN: 1949-2553; 1949-2553
• DOI: 10.18632/oncotarget.12236

Actin microfilaments and microtubules are both highly dynamic cytoskeleton components implicated in a wide range of intracellular processes as well as cell-cell and cell-substrate interactions. The interactions of actin filaments with the microtubule system play an important role in the assembly and maintenance of 3D cell structure. Here we demonstrate that cytoplasmic actins are differentially distributed in relation to the microtubule system. LSM, 3D-SIM, proximity ligation assay (PLA) and co-immunoprecipitation methods applied in combination with selective depletion of β- or γ-cytoplasmic actins revealed a selective interaction between microtubules and γ-, but not β-cytoplasmic actin via the microtubule +TIPs protein EB1. EB1-positive comet distribution analysis and quantification have shown more effective microtubule growth in the absence of β-actin. Our data represent the first demonstration that microtubule +TIPs protein EB1 interacts mainly with γ-cytoplasmic actin in epithelial cells.